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A tri state mechanism for oxygen release in fish hemoglobin: Using Barbus sharpeyi as a model | ||
| Molecular Biology Research Communications | ||
| مقاله 2، دوره 3، شماره 2، شهریور 2014، صفحه 101-113 اصل مقاله (646.05 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2014.2026 | ||
| نویسندگان | ||
| Mohammad Reza Dayer* 1؛ Mohammad Saaid Dayer2؛ Ali Akbar Moosavi-Movahedi3 | ||
| 1Department of Biology, Faculty of Science, Shahid Chamran University, Ahvaz, Iran | ||
| 2Department of Parasitology and Medical Entomology, Tarbiat Modares University, Tehran, Iran | ||
| 3Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran | ||
| چکیده | ||
| Hemoglobin is a porphyrin containing protein with an a2b2 tetrameric structure and like other porphyrin compounds shows spectral behavior of species specific characteristics. Researchers tend to relate bands in the hemoglobin spectra to certain structural and/or functional features. Given the fact that hemoglobin is the main oxygen carrier in animals functioning through the Oxy«Deoxy equilibrium, the determination of oxy and deoxy conformations of hemoglobins of different animals may shed light on their oxygen binding properties. Absorption spectra at 280 and 373nm have been widely used to quantitate the formation of hemoglobin deoxy conformation. In the present work, however, we used an optical density ratio of OD373/OD280 as an index for deoxy formation. This ratio was determined for Barbus sharpeyi and human hemoglobins at different SDS concentrations, pH levels and temperatures to compare them from a structure-function point of view. Our data showed that under low concentrations of SDS (Barbus sharpeyi hemoglobin folds in a tri-state pattern while human hemoglobin folds through a two-state phenomenon. This finding indicates that in contrast to those of other non aquatic animals, the hemoglobin of Barbus sharpeyi has a loosely folded tetrameric structure with remarkably more oxygen affinity | ||
| کلیدواژهها | ||
| Barbus sharpeyi؛ Hemoglobin؛ Tri State Mechanism | ||
| مراجع | ||
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