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Expression profile of cold shock protein genes in goats (Capra hircus) during different seasons | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 2، دوره 15، شماره 1 - شماره پیاپی 46، 2014، صفحه 7-12 اصل مقاله (161.15 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2014.1974 | ||
| نویسندگان | ||
| M. Gupta1؛ S. S. Dangi1؛ D. Maurya1؛ V. P. Yadav2؛ V. S. Chouhan3؛ R. K. Mahapatra3؛ G. Singh3؛ A. Mitra4؛ M. Sarkar* 3 | ||
| 1MVSc in Veterinary Physiology, Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India | ||
| 2MSc in Biotechnology, Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India | ||
| 3Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India | ||
| 4Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India | ||
| چکیده | ||
| The study was conducted to demonstrate expression profile of cold shock protein genes; cold inducible RNA binding protein (CIRP) and RNA binding motif protein 3 (RBM3) in goats (Capra hircus) during different seasons to check the gene expression in different thermal conditions. Blood samples were collected from healthy goats of tropical and temperate region during peak winter, moderate season and peak summer. Goats were divided in three (n=6 in each group) age groups- (I) up to 2 years, (II) 2 to 5 years and (III) above 5 years. RNA isolation was done from separated peripheral blood mononuclear cells (PBMCs) by Trizol reagent. Real-time PCR was applied to investigate mRNA expression of examined factors. The mRNA expression of CIRP and RBM3 was higher in all age groups during peak winter season as compared to peak summer season in both tropical and temperate region goats. To conclude, our results demonstrated expression of CIRP and RBM3 mRNAs in caprine PBMCs and increased expression of these genes during winter season could possibly play a significant role to ameliorate deleterious effects of cold stress to maintain cellular homeostasis in goats. | ||
| کلیدواژهها | ||
| Goat؛ cold stress؛ CIRP؛ RBM3؛ PBMCs | ||
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