| تعداد نشریات | 24 |
| تعداد شمارهها | 851 |
| تعداد مقالات | 7,575 |
| تعداد مشاهده مقاله | 13,413,762 |
| تعداد دریافت فایل اصل مقاله | 11,580,455 |
Up-regulation of plasma membrane H+-ATPase under salt stress may enable Aeluropus littoralis to cope with stress | ||
| Molecular Biology Research Communications | ||
| مقاله 6، دوره 3، شماره 1، خرداد 2014، صفحه 67-75 اصل مقاله (141.96 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2014.1955 | ||
| نویسندگان | ||
| Hosna Olfatmiri؛ Abbas Alemzadeh* ؛ Zahra Zakipour | ||
| Department of Crop Production and Plant Breeding, College of Agriculture, Shiraz University, Shiraz, Iran | ||
| چکیده | ||
| Plasma membrane H+-ATPase is a major integral membrane protein with a role in various physiological processes including abiotic stress response. To study the effect of NaCl on the expression pattern of a gene encoding the plasma membrane H+-ATPase, an experiment was carried out in a completely random design with three replications. A pair of specific primers was designed based on the sequence of the gene encoding plasma membrane H+-ATPase in Aeluropus littoralis to amplify a 259 bp fragment from the target gene by PCR. A gene encoding actin was used as reference gene to normalize the expression level of the target gene. A pair of specific primers was designed to amplify a 157 bp fragment from the actin gene by PCR. Plants were treated with different concentrations of NaCl, 0, 50, 100, 150, 200, 250, 500 and 1000 mM, for two days. Our results showed that the expression level of the plasma membrane H+-ATPase gene increased dramatically at 500 mM and then decreased with increasing concentrations of NaCl. The results also indicated that the leaves of plants, were treated with high concentrations of NaCl changed morphologically, but those grown under low concentrations of NaCl as well as the control plants did not show morphological changes in their leaves. Our results suggest a relation between morphological changes of treated plants and the expression level of the plasma membrane H+-ATPase gene in Aeluropus littoralis. | ||
| کلیدواژهها | ||
| Gene expression؛ Gouan, NaCl؛ Proton pump؛ Real-time PCR | ||
| مراجع | ||
|
1.Chen LJ, Wuriyanghan H, Zhang YQ, Duan KX, Chen HW, Li QT, Lu X, He SJ, Ma B, Zhang WK, Lin Q, Chen SY, Zhang JS. An S-domain receptor-like kinase, OsSIK2, confers abiotic stress tolerance and delays dark-induced leaf senescence in rice. Plant Physiol 2013;163:1752-1765.
2.Zhao Y, Pan Z, Zhang Y, Qu X, Zhang Y, Yang Y, Jiang X, Huang S, Yuan M, Schumaker KS, Guo Y. The actin-related protein2/3 complex regulates mitochondrial-associated calcium signaling during Salt stress in Arabidopsis. Plant Cell 2013;25:4544-4559.
3.Zhu JK. Regulation of ion homeostasis under salt stress. Curr Opin Plant Biol 2003;6:441-445.
4.Morsomme P, Boutry M. The plant plasma membrane H+-ATPase: structure, function and regulation. Biochim Biophys Acta 2000;1465:1-16.
5.Hasegawa PM, Bressan RA, Zhu JK, Bohnert HJ. Plant cellular and molecular responses to high salinity. Annu Rev Plant Physiol Plant Mol Biol 2000;51:463-499.
6.Zhang Y, Wang L, Liu Y, Zhang Q, Wei Q, Zhang W. Nitric oxide enhances salt tolerance in maize seedlings through increasing activities of proton-pump and Na+/H+ antiport in the tonoplast. Planta 2006;224:545-555.
7.López Pérez L, Martínez-Ballesta MC, Maurel C, Carvajal M. Changes in plasma membrane lipids, aquaporins and proton pump of broccoli roots, as an adaptation to salinity. Phytochemistry 2009;70:492-500.
8.Golldack D, Dietz KJ. Salt-induced expression of the vacuolar H+-ATPase in the common ice plant is developmentally controlled and tissue specific. Plant Physiol 2001;125:1643-1654.
9.Sibole JV, Cabot C, Michalke W, Posehenrieder C, Barceló J. Relationship between expression of the PM H+-ATPase, growth and ion partitioning in the leaves of salt-treated Medicago species. Planta 2005;221:557-566.
10.Barhoumi Z, Djebali W, Chaibi W, Abdelly C, Smaoui A. Salt impact on photosynthesis and leaf ultrastructure of Aeluropus littoralis. J Plant Res 2007; 120:529-537.
11.Ayala F, O’Leary JW, Schumaker KS. Increased vacuolar and plasma membrane H+-ATPase activities in Salicornia bigelovii Torr. in response to NaCl. J Exp Bot.1996; 47:25-32.
12.Muramatsu Y, Harada A, Ohwaki Y, Kasahara Y, Takagi S, Fukuhara T. Salt-tolerant ATPase activity in the plasma membrane of the marine angiosperm Zostera marina L. Plant Cell Physiol 2002;43:1137-1145.
13.Pak JY, Fukuhara T, Nitta T. Discrete subcellular localization of membrane-bound ATPase activity in marine angiosperms and marine algae. Planta 1995;196:15-22.
14.Fukuhara T, Pak JY, Ohwaki Y, Tsujimura H, Nitta T. Tissue-specific expression of the gene for a putative plasma membrane H+-ATPase in a seagrass. Plant Physiol 1996;110:35-42.
15.Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plantarum 1962;15:437-497.
16.Alemzadeh A, Fujie M, Usami S, Takashi Y. Isolation of high-quality RNA from high-phenolic tissues of eelgrass (Zostera marina L.) by keeping temperature low. Plant Mol Biol Rep 2005;23:421a-421h.
17.Tanou G, Molassiotis A, Diamantidis G. Induction of reactive oxygen species and necrotic death-like destruction in strawberry leaves by salinity. Environ Exp Bot 2009;65:270-281.
18.Ungar IA. Effect of salinity on seed germination, growth and ion accumulation of Atriplex patula (Chenopodiaceae). Am J Bot 1996;83:604-647.
19.Koyro HW. Effect of salinity on growth, photosynthesis, water relations and solute composition of potential cash crop halophyte Plantago coronopus (L.). Environ Exp Bot 2006;56:136-146.
20.Kim DW, Shibato J, Agrawal GK, Fujihara S, Iwahashi H, Kim DH, Shim IS, Rakwal R. Gene transcription in the leaves of rice undergoing salt-induced morphological changes (Oryza sativa L.). Mol Cells 2007;24:45-59.
21.Niu X, Zhu JK, Narasimhan ML, Bressan RA, Hasegawa PM. Plasma-membrane H+-ATPase gene expression is regulated by NaCl in cells of the halophyte Atriplex nummularia L. Planta 1993;190:433-438.
22.Silva P, Gerós H. Regulation by salt of vascular H+-ATPase and H+-pyrophosphatase activities and Na+/H+ exchange. Plant Sig Bihav 2009;4:718-726.
23.Gao F, Gao Q, Duan XG, Yue GD, Yang AF, Zhang JR. Cloning of an H+-ATPase gene from Thellungiella halophila and its heterologous expression to improve tobacco salt tolerance. J Exp Bot 2006;57:3259-3270.
24.Olias R, Eljakaoui Z, Li J, Alvarez de Morales P, Marin-Manzano MC, Pardo JM, Belver A. The plasma membrane Na+/H+ antiporter SOS1 is essential for salt tolerance in tomato and affects the partitioning of Na+ between plant organs. Plant Cell Environ 2009;32:904-916.
25.Zhang GH, Li-Jia An QS, Wu S. Characterization and expression of a vacuolar Na+/H+ antiporter gene from the monocot halophyte Aeluropus littoralis. Plant Physiol Biochem 2008;46:117-126.
26.Ji H, Pardo JM, Batelli G, Oosten MJV, Bressan RA, Li X. The salt overly sensitive (SOS) pathway: established and engineering roles. Mol Plant 2013;279:812-819.
27.Hahnenberger KM, Jia Z, Young PG. Functional expression of the Saccharomyces pombe Na+/H+ antiporter gene, sod2, in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 1996;93:5031-5036.
28.Sondergaard TE, Schulz A, Palmgren MG. Energization of transport processes in plants: roles of the plasma membrane H+-ATPase. Plant Physiol 2004;136:2475-2482.
29. Hager A, Debus G, Edel HG, Stransky H, Serrano R. Auxin induces exocytosis and the rapid synthesis of a high-turnover pool of plasma membrane H+-ATPase. Planta 1991;185:527-537. | ||
|
آمار تعداد مشاهده مقاله: 4,269 تعداد دریافت فایل اصل مقاله: 4,796 |
||