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Cellulase Production by Trichoderma reesei using Sugar Beet Pulp | ||
| Iran Agricultural Research | ||
| مقاله 9، دوره 25.26، شماره 1.2، خرداد 2007، صفحه 107-116 اصل مقاله (223.63 K) | ||
| نوع مقاله: Full Article | ||
| شناسه دیجیتال (DOI): 10.22099/iar.2008.190 | ||
| نویسندگان | ||
| M. Moosavi-Nasab* ؛ M. Majdi-Nasab | ||
| Department of Food Science and Technology, College of Agriculture, Shiraz University, Shiraz, I. R. Iran | ||
| چکیده | ||
| Cellulase production by the fungus Trichoderma reesei was studied using sugar beet pulp (SBP) as a substrate. The subculture medium was a salt solution consisting of KH2PO4, CaCl2, etc. Fungal cells were sub-cultured in an orbital shaker (180 rpm) at 30°C for 1-2 generations (two days for each generation) and were then used as an inoculum. Exponential cells were inoculated into a medium containing SBP. Data showed that cellulose in SBP has a potential to be used as a substrate to produce cellulase. The effect of cellulose concentration on cellulase production was investigated. Using the medium composition (the mixture of chemical salts and SBP substrate) and a fermentation time of 4-6 days, a maximum cellulase activity of 0.46 IU/ml of filter paper activity was obtained. | ||
| کلیدواژهها | ||
| Cellulase؛ Sugar beet pulp؛ Trichoderma reesei | ||
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