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GSTF1 Gene Expression Analysis in Cultivated Wheat Plants under Salinity and ABA Treatments | ||
| Molecular Biology Research Communications | ||
| مقاله 2، دوره 3، شماره 1، خرداد 2014، صفحه 9-19 اصل مقاله (109.26 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2014.1889 | ||
| نویسندگان | ||
| Ali Niazi* 1؛ Amin Ramezani2؛ Ali Dinari3 | ||
| 1Head of Biotechnology Institute, Shiraz University, Shiraz, Iran | ||
| 2Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 3Biotechnology Institute, Shiraz University, Bajgah, Shiraz, Iran | ||
| چکیده | ||
| Most plants encounter stress such as drought and salinity that adversely affect growth, development and crop productivity. The expression of the gene glutathione-s-transferases (GST) extends throughout various protective mechanisms in plants and allows them to adapt to unfavorable environmental conditions. GSTF1 (the first phi GSTFs class) gene expression patterns in the wheat cultivars Mahuti and Alamut were studied under salt and ABA treatments using a qRT-PCR technique. Results showed that gene expression patterns were significantly different in these two cultivars. Data showed that in Mahuti, there was an increase of transcript accumulation under salt and ABA treatments at 3h, 10h and 72h respectively. In Alamut, however, the pattern of transcript accumulation was different; the maximum was at 3h. In contrast, there were no significant differences observed between the cultivars for GSTF1 gene expression profiles at three levels of NaCl concentration (50, 100, and 200 mM) or in ABA (Abscisic Acid) treatment. It is likely that difference of gene expression patterns between the cultivars (Mahuti as a salt tolerant cultivar and Alamut as a salt sensitive cultivar) is due to distinct signaling pathways which activate GSTF1 expression. Lack of a significant difference between the GSTF1 gene expression profile under salt and ABA treatments suggests that the GSTF1 gene is not induced by stress stimuli. Of course it is possible that other levels of NaCl and ABA treatments cause a change in the GSTF1 gene. | ||
| کلیدواژهها | ||
| ABA؛ Salinity؛ GSTF1؛ qRT-PCR؛ Wheat | ||
| مراجع | ||
|
1.Abebe T, Guenzi AC, Martin B, Cushman JC. Tolerance of mannitol-accumulating transgenic wheat to water stress and salinity. Plant Physiol 2003;131:1748-1755.
2.Zamani Babgohari M, Niazi A, Moghadam AA, Deihimi T, Ebrahimie E. Genome-wide analysis of key salinity-tolerance transporter (HKT1;5) in wheat and wild wheat relatives (A and D genomes). In Vitro Cell Dev Biol Plant 2013;49:97-106.
3.Boyer JS. Plant Productivity and Environment. Science 1982;218:443-448.
4.Niu X, Bressan RA, Hasegawa PM, Pardo JM. Ion homeostasis in NaCl stress environments. Plant Physiol 1995;109:735-742.
5.Wang WX, Vinocur B, Altman A. Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta 2003;218:1-14.
6.Moghadam AA, Ebrahimie E, Taghavi SM, Niazi A, Djavaheri M. Isolation and in silico functional analysis of MtATP6, a 6-kDa subunit of mitochondrial F1F0-ATP synthase, in response to abiotic stress. Genet Mol Res 2012;11:3547-3567.
7.Seppanen MM, Cardi T, Hyokki MB, Pehu E. Characterisation and expression of cold-induced gluathione S-transferase in freezing tolerant Solanum commersonii, sensitive S. tuberosum and their interspecific somatic hybrids. Plant Sci 2000;153: 125-133.
8.Uquillas C, Letelier I, Blanco F, Jordana X, Holuigue L. NPR1-independent activation of immediate early salicylic acid responsive genes in Arabidopsis. Mol Plant Microbe Interac 2004;17:34-42.
9.Zhao f, Zhang H. Expression of Suaeda salsa glutathione S-transferase in transgenic rice resulted in a different level of abiotic stress resistance. J Agr Science 2006;144: 547-554.
10.Zhu JK. Plant salt tolerance. Trends Plant Sci 2001;6:66-71.
11.Hasegawa PM, Bressan RA, Zhu JK, Bohnert HJ. Plant cellular and molecular responses to high salinity. Annu Rev Plant Physiol Plant Mol 2000;51:463-499.
12.Gonneau M, Pagant S, Brun F, Laloue M. Photoaffinity labelling with the cytokinin agonist azido-CPPU of a 34 kDa peptide of the intracellular pathogenesis-related protein family in the moss Physcomitrella patens. Plant Mol Biol 2001;46:539-548.
13.Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2- DDCT method. Methods 2001; 25: 402-408.
14.Mauch F, Dudler R. Differential induction of distinct glutathione transferases of wheat by xenobiotics and by pathogen attack. Plant Physiol 1993;102:1193-1201.
15.Frova C. The plant glutathione trasnferase gene family: genomic structure, functions, expression and evolution. Physiol Plant 2003;119:469-479.
16.Piero AR, Mercurio V, Puglisi I, Petrone G. Gene isolation and expression analysis of two distinct sweet orange Citrus sinensis L. (Osbeck) tau-type glutathione transferases. Gene 2009;443:143-150.
17.Breusegem FV, Vranova E, Dat JF, Inze D. The role of active oxygen species in plant signal transduction. Plant Sci 2001;161:405-414.
18.Asada K. The water–water cycle in chloroplasts: scavenging of active oxygen’s and dissipation of excess photons. Annu Rev Plant Physiol Plant Mol Biol 1999; 50:601-639.
19.Dixon DP, Edwards R. Selective binding of glutathione conjugates of fatty acid derivatives by plant glutathione transferases. J Biol Chem 2009;284:21249-21256.
20.Dixon DP, Lapthorn A, Edwards R. Plant glutathione transferases. Genome Biol 2002;3:1-10.
21.Luan S. Signalling drought in guard cells. Plant Cell Environ 2002;25:229-237.
22.Droog FN, Hooykaas PJ, Van der Zaal BJ. 2,4-Dichlorophenoxyacetic acid and related chlorinated compounds inhibit two auxin-regulated type-III tobacco glutathione S-transferases. Plant Physiol 1995;107:1139-1146.
23.Edwards R, Dixon DP. The role of glutathione transferases in herbicide metabolism. In: Cobb AH, Kirkwood RC. (eds), Herbicides and their mechanisms of action, Sheffield Academic Press, Ltd, Sheffield, 2000; pp. 38-71.
24.Jain M, Nijhawan A, Tyagi AK, Khurana JP. Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. Biochem Biophy Res 2006;345:646-651.
25.Edwards R, Dixon DP. Plant glutathione transferases. Methods Enzymol 2005;41: 169-86.
26.Dixon DP, Lapthorn A, Madesis P, Mudd EA, Day A, Edwards R. Binding and glutathione conjugation of porphyrinogens by plant glutathione transferases. J Biol Chem 2008;283:20268-20276.
27.Blumwald E, Aharon GS, Apse MP. Sodium transport in plant cells. Biochimica Et Biophysica Acta 2000;1465:140-151.
28.Nicot N, Hausman JF, Hoffmann L, Evers D. Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress. J Exp Bot 2005;56:2907-2914.
29.Galle A, Csiszar J, Secenji M, Guoth A, Cseuz L, Tari I, Gyorgyey J, Erdei L. Glutathione transferase activity and expression patterns during grain filling in flag leaves of wheat genotypes differing in drought tolerance: Response to water deficit. J Plant Physiol 2009;166:1878-1891.
30.Jones AM, Thomas V, Truman B, Lilley K, Mansfield J, Grant M. Specific changes in the Arabidopsis proteome in response to bacterial challenge: differentiating basal and R-gene mediated resistance. Phytochemistry 2004;65:1805-1816
31.Caldana C, Scheible WR, Roeber BM, Ruzicic S. A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors. Plant Methods 2007;3:7.
32.Hernandez JA, Olmos E, Corpas FJ, Sevilla F, Del-Rio LA. Salt-induced oxidative stress in chloroplasts of pea plants. Plant Sci 1995;105:151-167.
33.Moghadam AA, Ebrahimie E, Taghavi SM, Niazi A, Zamani Babgohari M, Deihimi T, Mohammad D, Ramezani A. How the nucleus and mitochondria communicate in energy production during stress: nuclear MtATP6, an early-stress responsive gene, regulates the mitochondrial F1F0-ATP synthase complex Mol Biotechnol 2013; 54:756-69.
34.Kreps JA, Wu Y, Chang HS, Zhu T, Wang X, Harper JF. Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress. Plant Physiol 2002;130: 2129-2141.
35.Larionov A, Krause A, Miller W. A standard curve based method for relative real time PCR data processing. BMC Bioinform 2005; doi:10.1186/1471-2105-6-62.
36.Marrs KA. The function and regulation of glutathione S-transferases in plants. Annu Rev Plant Phys 1996;47:127-158.
37.Seki M, Narusaka M, Abe H, Kasuga M, Yamaguchi-Shinozaki K, Carninci P, Hayashizaki Y, Shinozaki K. Monitoring the expression pattern of 1300 Arabidopsis gene under drought and cold stresses by using a full-length cDNA microarry. Plant Cell 2001;13:61-72.
38.Chen W, Chao G, Singh KB. The promoter of a H2O2-inducible, Arabidopsis glutathione S-transferase gene contains closely linked OBF- and OBP1-binding sites. Plant J 1996;10:955-966.
39.Ghavami F, Malboobi MA, Ghannadha MR, Lohrasebi T, Yazdi-Samadi B, Mozaffari J, et al. Up-regulation of succinate dehydrogenase and prophobilinogen deaminase in response to high salt concentration in wheat. Iranian J Agri Sci 2006; 36:1437-1444.
40.Serrano R, Mulet JM, Rios G, Marquez JA, de Larrinoa IF, Leube MP, Mendizabal I, Pascual-Ahuir A, Proft M, Ros R, Montesinos C. A glimpse of the mechanisms of ion homeostasis during salt stress. J Exp Bot 1999;50:1023-1036.
41.Dixon DA, Edwards R. Glutathione Transferases. Am Soc Plant Biologist 2010; 10.1199/tab.0131.
42.Wang WX, Vinocur B, Shoseyov O, Altman A. Biotechnology of plant osmotic stress tolerance: physiological and molecular considerations. Acta Hort 2001;560: 285-292.
43.Dixon DP, Skipsey M, Grundy NM, Edwards R. Stress induced protein S-glutathionylation in Arabidopsis. Plant Physiol 2005;138:2233-2244.
44.DeRidder BP; Dixon DP, Beussman DJ, Edwards R, Goldsbrough PB. Induction of glutathione S-transferases in Arabidopsis by herbicide safeners. Plant Physiol 2002; 130:1497-1505.
45.Dinari A, Niazi A, Afsharifar AR, Ramezani A. Identification of upregulated genes under cold stress in cold-tolerant chickpea using the cDNA-AFLP approach. PLOS ONE 2013;e52757.
46.Ji W, Zhu Y, Li Y, Yang L, Zhao X, Cai H, Bai X. Over-expression of a glutathione S-transferase gene, GsGST, from wild soybean (Glycine soja) enhances drought and salt tolerance in transgenic tobacco. Biotechnol Lett 2010;32:1173-1179.
47.Smith AP, Nourizadeh SD, Peer WA, Xu J, Bandyopadhyay A, Murphy AS, Goldsbrough PB. Arabidopsis At-GSTF2 is regulated by ethylene and auxin, and encodes a glutathione S-transferase that interacts with flavonoids. Plant J 2003;36: 433-442.
48.Ramezani A, Niazi A, Moghadam AA, Zamani Babgohari M, Deihimi T, Ebrahimi M, Akhtardanesh H, Ebrahimie E. Quantitative expression analysis of TaSOS1 and TaSOS4 genes in cultivated and wild wheat plants under salt stress. Mol Biotechnol 2013;53:189-197.
49.Xu FX, Lagudah ES, Moose SP, Riechers DE. Tandemly duplicated safener-induced glutathione S-transferase genes from Triticum tauschii contribute to genome-and organ specific expression in hexaploid wheat. Plant Physiol 2002;130:362-373.
50.Kerepesi I, Galiba G. Osmotic and salt stress-induced alteration in soluble carbohydrate content in wheat seedlings. Crop Sci 2000;40:482-487.
51.Ryu HY, Kim SY, Park HM, You JY, Kim BH, Lee JS,NamKH. Modulations of AtGSTF10 expression induce stress tolerance and BAK1-mediated cell death. Biochem Biophys Res Commun 2009;379:417-422.
52.Seki M, Ishida J, Narusaka M, Fujita M, Nanjo T, Umezawa T, Kamiya A, Nakajima M, Enju A, Sakurai T, Satou M, Akiyama K, Yamaguchi-Shinozaki K, Carnine P, Kawai J, Hayashizaki Y, Shinozaki K. Monitoring the expression pattern of around 7000 Arabidopsis genes under ABA treatments using a full-length cDNA microarray. Funct Integr Genomics 2002;2:282-291.
53.Zhang J, Jia W, Yang J, Ismail AM. Role of ABA in integrating plant responses to drought and salt stresses. Field Crops Res 2006;97:111-119.
54.Qi YC, Liu WQ, Qiu LY, Zhang SM, Ma L, Zhang H. Overexpression of glutathione s-transferase gene increases salt tolerance of Arabidopsis. Russian J Plant Physiol 2010;57:233-240. | ||
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