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Hiremath, C., Jhala, M. K., Bhanderi, B. B., Joshi, C. G.. (1392). Cloning and sequence analysis of VP1, VP2 and VP3 genes of Indian chicken anemia virus. , 14(4), 354-357. doi: 10.22099/ijvr.2013.1835
C. Hiremath; M. K. Jhala; B. B. Bhanderi; C. G. Joshi. "Cloning and sequence analysis of VP1, VP2 and VP3 genes of Indian chicken anemia virus". , 14, 4, 1392, 354-357. doi: 10.22099/ijvr.2013.1835
Hiremath, C., Jhala, M. K., Bhanderi, B. B., Joshi, C. G.. (1392). 'Cloning and sequence analysis of VP1, VP2 and VP3 genes of Indian chicken anemia virus', , 14(4), pp. 354-357. doi: 10.22099/ijvr.2013.1835
Hiremath, C., Jhala, M. K., Bhanderi, B. B., Joshi, C. G.. Cloning and sequence analysis of VP1, VP2 and VP3 genes of Indian chicken anemia virus. , 1392; 14(4): 354-357. doi: 10.22099/ijvr.2013.1835

Cloning and sequence analysis of VP1, VP2 and VP3 genes of Indian chicken anemia virus

Iranian Journal of Veterinary Research
مقاله 13، دوره 14، شماره 4 - شماره پیاپی 45، 2013، صفحه 354-357    اصل مقاله (118.12 K)
نوع مقاله: Short paper
شناسه دیجیتال (DOI): 10.22099/ijvr.2013.1835
نویسندگان
C. Hiremath1؛ M. K. Jhala2؛ B. B. Bhanderi* 2؛ C. G. Joshi3
1MVSc in Veterinary Microbiology, Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388001, Gujarat, India
2Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388001, Gujarat, India
3Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388001, Gujarat, India
چکیده
Chicken anemia virus was detected by PCR in tissue samples collected from poultry flocks in Gujarat,
India. The VP1, VP2 and VP3 gene sequences of CAV from Anand, Gujarat were obtained after cloning the
PCR products in pDrive cloning vector. Nucleotide sequence alignment with other CAV sequences
demonstrated overall identity of 95-98.8%, 98.8-99.8% and 98.8-100% for VP1, VP2 and VP3 regions,
respectively. Deduced amino acid sequences revealed 91.7-99.7%, 99-100% and 97.3-100% homologies for
VP1, VP2 and VP3 proteins, respectively, indicating high level of genome conservation. Further, placement
of critical nucleotides and amino acids at particular positions indicated that Anand CAV is possibly of more
pathogenic potential. The CAV isolates were phylogenetically grouped together independent of their
geographic origin.
کلیدواژه‌ها
Chicken anemia virus؛ Chicken infectious anemia؛ India
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