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Development of a recombinant protein-based dot-blot hybridization assay for the detection of antibody to chicken infectious bronchitis virus | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 12، دوره 14، شماره 4 - شماره پیاپی 45، 2013، صفحه 350-353 اصل مقاله (203.94 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2013.1834 | ||
| نویسندگان | ||
| H. Akrami1؛ A. Hedayati2؛ M. Farshian2؛ G. Haqshenas* 3 | ||
| 1Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran | ||
| 2Iranian Veterinary Organization, Tehran, Iran | ||
| 3National Research Center for Genetic Engineering and Biotechnology (NRCGEB), Tehran, Iran | ||
| چکیده | ||
| Nucleocapsid (N) protein of infectious bronchitis virus (IBV), one of the viral structural proteins, induces strong antibody response in natural infection. In this study, a simple, recombinant N protein-based dot-blot test was developed to serologically examine chicken serum samples for the presence of IBV antibody. Initially, 72 serum samples were tested for the presence of IBV antibody using a commercial enzyme linked immunosorbent assay (ELISA) kit. Forty six IBV positive serum samples (group A) produced strong signals in the dot-blot assay. Seven negative serum samples (group B) produced weak but specific signals using the dot-blot assay in conjunction with Western blot analysis. The remaining 19 samples (group C) from IBV negative specific pathogen free (SPF) chickens did not produce visible signals using the dot-blot assay. In conclusion, the above results suggest that the dot-blot assay is a reliable, sensitive, and specific test for serological detection of IBV positive chickens. | ||
| کلیدواژهها | ||
| Infectious bronchitis virus؛ Dot-blot hybridization؛ Nucleocapsid protein | ||
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