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Singh, S. V., Singh, P. K., Gupta, S., Chaubey, K. K., Singh, B., Kumar, A., Singh, A. V., Kumar, N.. (1392). Comparison of microscopy and blood-PCR for the diagnosis of clinical Johne’s disease in domestic ruminants. , 14(4), 345-349. doi: 10.22099/ijvr.2013.1833
S. V. Singh; P. K. Singh; S. Gupta; K. K. Chaubey; B. Singh; A. Kumar; A. V. Singh; N. Kumar. "Comparison of microscopy and blood-PCR for the diagnosis of clinical Johne’s disease in domestic ruminants". , 14, 4, 1392, 345-349. doi: 10.22099/ijvr.2013.1833
Singh, S. V., Singh, P. K., Gupta, S., Chaubey, K. K., Singh, B., Kumar, A., Singh, A. V., Kumar, N.. (1392). 'Comparison of microscopy and blood-PCR for the diagnosis of clinical Johne’s disease in domestic ruminants', , 14(4), pp. 345-349. doi: 10.22099/ijvr.2013.1833
Singh, S. V., Singh, P. K., Gupta, S., Chaubey, K. K., Singh, B., Kumar, A., Singh, A. V., Kumar, N.. Comparison of microscopy and blood-PCR for the diagnosis of clinical Johne’s disease in domestic ruminants. , 1392; 14(4): 345-349. doi: 10.22099/ijvr.2013.1833

Comparison of microscopy and blood-PCR for the diagnosis of clinical Johne’s disease in domestic ruminants

Iranian Journal of Veterinary Research
مقاله 11، دوره 14، شماره 4 - شماره پیاپی 45، 2013، صفحه 345-349    اصل مقاله (163.76 K)
نوع مقاله: Short paper
شناسه دیجیتال (DOI): 10.22099/ijvr.2013.1833
نویسندگان
S. V. Singh* 1؛ P. K. Singh2؛ S. Gupta1؛ K. K. Chaubey1؛ B. Singh1؛ A. Kumar1؛ A. V. Singh2؛ N. Kumar1
1Microbiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, PO-Farah, Pin-281122, District Mathura, Uttar Pradesh, India
2National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Tajganj, Agra-282 001, India
چکیده
Microscopy as “field based test” was compared with IS900 blood PCR for the diagnosis and estimation
of prevalence of Mycobacterium avium subspecies paratuberculosis (MAP) in domestic livestock with
clinical Johne’s disease (JD). Of 252 animals screened, 39.3 and 13.1% were positive by faecal microscopy
and blood PCR, respectively. Proportional agreement between “microscopy” and blood PCR was substantial.
1262 faecal samples from animals suspected of clinical JD were screened by microscopy in 2009-2010 and
41.3% were positive. Prevalence of MAP was highest in cattle (61.8%), followed by goats (41.3%), sheep
(33.4%) and buffaloes (15.7%). Faecal microscopy was cost effective, easy to adopt and repeatable for the
screening of domestic ruminant population against MAP infection.
کلیدواژه‌ها
paratuberculosis؛ Ziehl Neelsen staining؛ IS900 PCR؛ India
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