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The effect of seminal plasma on the quality of coated ram frozen-thawed spermatozoa | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 5، دوره 14، شماره 4 - شماره پیاپی 45، 2013، صفحه 305-312 اصل مقاله (68.03 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2013.1827 | ||
| نویسندگان | ||
| M. Roostaei-Ali Mehr* 1؛ F. Sharafi2 | ||
| 1Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| 2Graduated from Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| چکیده | ||
| Two experiments were designed to examine the effects of crude seminal plasma (CSP) exposure of ejaculated and epididymal spermatozoa before freezing. Experiment 1, two consecutive ejaculates were collected (n=4) within 5 min, the second one was carried out in a tube containing Fiser extender (coated spermatozoa); by centrifugation, seminal plasma was removed from coated ejaculates. The pellets were split into three parts and 0, 50 and 100% CSP (v/v) was added and they were frozen. Samples were thawed and incubated at 37°C for 6 h. The highest progressive motility (10.41%) and the highest hypo-osmotic responses (12.62%) were found for the coated spermatozoa without CSP at 6 (P<0.05). The maximum viability was found for coated spermatozoa without CSP at 0 (29.4%) and 6 (17%, P<0.05). Experiment 2, epididymal spermatozoa were recovered (n=6), pooled and split into three fractions and 0, 50 and 100% CSP and the diluents were added, after that they were frozen. Thawed epididymal spermatozoa were incubated at 37°C for 6 h. The highest (33.3%) and lowest (25%) progressive motility were found for the epididymal spermatozoa without CSP and the epididymal spermatozoa with 100% CSP at 0, respectively (P<0.05). The highest (19.37%) and the lowest (9.38%) viability were related to the epididymal spermatozoa with 0 and 100% CSP at 6, respectively (P<0.05). Under the conditions of the current study, the addition of CSP to the ram epididymal and coated spermatozoa strengthened the detrimental effect of the freezing procedure. | ||
| کلیدواژهها | ||
| Cryopreservation؛ Sperm coating؛ Seminal plasma؛ Ram؛ Spermatozoa | ||
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