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Effect of follicular size on in vitro maturation, fertilization and culture of sheep embryos | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 4، دوره 14، شماره 4 - شماره پیاپی 45، 2013، صفحه 299-304 اصل مقاله (115.11 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2013.1826 | ||
| نویسندگان | ||
| A. R. Wani1؛ M. Z. Khan2؛ K. A. Sofi2؛ A. A. Malik* 2؛ F. A. Lone2؛ F. A. Bhat1 | ||
| 1MVSc Student in Animal Reproduction, Department of Animal Reproduction, Gynaecology and Obstetrics, Faculty of Veterinary Sciences and Animal Husbandry Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, India | ||
| 2Department of Animal Reproduction, Gynaecology and Obstetrics, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, 190 006, India | ||
| چکیده | ||
| A study was conducted to investigate the effect of follicular size (small, <2 mm diameter and large, ≥2 mm) on in vitro maturation, fertilization and culture of ovine oocytes. The ovaries (n=80) were collected from abattoirs in Srinagar city, India and transported in normal saline solution containing 50 μg gentamicin sulphate per ml in a thermos flask. The oocytes were collected by puncture technique and good and fair quality oocytes obtained were subjected to maturation in TCM-199 supplemented with epidermal growth factor (EGF) for 24 h under 5% CO2 and humidified atmosphere at 38.5°C. The matured oocytes were then subsequently subjected to fertilization and culturing was done for six days. The results revealed that the maturation rate of large follicle oocytes (85.20%) was higher than small follicle oocytes (52.44%, P=0.002). In vitro fertilization rates for small and large follicle oocytes were not significantly different (79.54 and 76.65%, respectively). However, cleavage and morula rates were higher in large follicle oocytes (58.69 and 34.13%) than small follicle oocytes (38.63 and 19.32%, respectively, P<0.05). In conclusion, there is higher developmental competence of oocytes from the large follicles than small follicles and the pre-maturation of oocyte occurring during follicular growth is essential for subsequent embryonic development in sheep. | ||
| کلیدواژهها | ||
| Cleavage؛ Follicular size؛ In vitro maturation؛ In vitro fertilization؛ Sheep | ||
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