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Wani, A. R., Khan, M. Z., Sofi, K. A., Malik, A. A., Lone, F. A., Bhat, F. A.. (1392). Effect of follicular size on in vitro maturation, fertilization and culture of sheep embryos. , 14(4), 299-304. doi: 10.22099/ijvr.2013.1826
A. R. Wani; M. Z. Khan; K. A. Sofi; A. A. Malik; F. A. Lone; F. A. Bhat. "Effect of follicular size on in vitro maturation, fertilization and culture of sheep embryos". , 14, 4, 1392, 299-304. doi: 10.22099/ijvr.2013.1826
Wani, A. R., Khan, M. Z., Sofi, K. A., Malik, A. A., Lone, F. A., Bhat, F. A.. (1392). 'Effect of follicular size on in vitro maturation, fertilization and culture of sheep embryos', , 14(4), pp. 299-304. doi: 10.22099/ijvr.2013.1826
Wani, A. R., Khan, M. Z., Sofi, K. A., Malik, A. A., Lone, F. A., Bhat, F. A.. Effect of follicular size on in vitro maturation, fertilization and culture of sheep embryos. , 1392; 14(4): 299-304. doi: 10.22099/ijvr.2013.1826

Effect of follicular size on in vitro maturation, fertilization and culture of sheep embryos

Iranian Journal of Veterinary Research
مقاله 4، دوره 14، شماره 4 - شماره پیاپی 45، 2013، صفحه 299-304    اصل مقاله (115.11 K)
نوع مقاله: Full paper (Original article)
شناسه دیجیتال (DOI): 10.22099/ijvr.2013.1826
نویسندگان
A. R. Wani1؛ M. Z. Khan2؛ K. A. Sofi2؛ A. A. Malik* 2؛ F. A. Lone2؛ F. A. Bhat1
1MVSc Student in Animal Reproduction, Department of Animal Reproduction, Gynaecology and Obstetrics, Faculty of Veterinary Sciences and Animal Husbandry Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, India
2Department of Animal Reproduction, Gynaecology and Obstetrics, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu and Kashmir, 190 006, India
چکیده
A study was conducted to investigate the effect of follicular size (small, <2 mm diameter and large, ≥2
mm) on in vitro maturation, fertilization and culture of ovine oocytes. The ovaries (n=80) were collected
from abattoirs in Srinagar city, India and transported in normal saline solution containing 50 μg gentamicin
sulphate per ml in a thermos flask. The oocytes were collected by puncture technique and good and fair
quality oocytes obtained were subjected to maturation in TCM-199 supplemented with epidermal growth
factor (EGF) for 24 h under 5% CO2 and humidified atmosphere at 38.5°C. The matured oocytes were then
subsequently subjected to fertilization and culturing was done for six days. The results revealed that the
maturation rate of large follicle oocytes (85.20%) was higher than small follicle oocytes (52.44%, P=0.002).
In vitro fertilization rates for small and large follicle oocytes were not significantly different (79.54 and
76.65%, respectively). However, cleavage and morula rates were higher in large follicle oocytes (58.69 and
34.13%) than small follicle oocytes (38.63 and 19.32%, respectively, P<0.05). In conclusion, there is higher
developmental competence of oocytes from the large follicles than small follicles and the pre-maturation of
oocyte occurring during follicular growth is essential for subsequent embryonic development in sheep.
کلیدواژه‌ها
Cleavage؛ Follicular size؛ In vitro maturation؛ In vitro fertilization؛ Sheep
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