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Cloning and secretory expression of VP2 gene of infectious bursal disease virus in eukaryotic cells | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 10، دوره 11، شماره 1 - شماره پیاپی 30، 2010، صفحه 72-77 اصل مقاله (123.76 K) | ||
| نوع مقاله: Short paper | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2010.178 | ||
| نویسندگان | ||
| Sh. Ghafari1؛ M. R. Seyfiabad Shapouri* 2؛ H. Moatamedi3؛ M. Roayaei3؛ H. Goudarzi4 | ||
| 1Graduated from Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| 2Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| 3Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| 4Department of Avian Medicine, Razi Vaccine and Serum Research Institute, Karaj, Iran | ||
| چکیده | ||
| VP2 gene coding region of a vaccinal strain (D78) of infectious bursal disease virus (IBDV) was cloned in a eukaryotic expression vector, pSec Tag2A. The gene was placed downstream of Ig κ chain leader sequence, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. The construct pSec Tag2A-VP2 was transfected in COS-7 cell line and the expression and secretion of VP2 was assessed by dot blotting and antigen capture ELISA. The antibody used in the immunological assays was a neutralizing monoclonal antibody (1A6) against VP2. Positive reaction with the antibody indicated the construct was functional with respect to expression and secretion of a native VP2. | ||
| کلیدواژهها | ||
| Infectious bursal disease؛ IBDV؛ VP2؛ Eukaryotic expression؛ COS-7 cell line | ||
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