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Ghafari, Sh., Seyfiabad Shapouri, M. R., Moatamedi, H., Roayaei, M., Goudarzi, H.. (1388). Cloning and secretory expression of VP2 gene of infectious bursal disease virus in eukaryotic cells. , 11(1), 72-77. doi: 10.22099/ijvr.2010.178
Sh. Ghafari; M. R. Seyfiabad Shapouri; H. Moatamedi; M. Roayaei; H. Goudarzi. "Cloning and secretory expression of VP2 gene of infectious bursal disease virus in eukaryotic cells". , 11, 1, 1388, 72-77. doi: 10.22099/ijvr.2010.178
Ghafari, Sh., Seyfiabad Shapouri, M. R., Moatamedi, H., Roayaei, M., Goudarzi, H.. (1388). 'Cloning and secretory expression of VP2 gene of infectious bursal disease virus in eukaryotic cells', , 11(1), pp. 72-77. doi: 10.22099/ijvr.2010.178
Ghafari, Sh., Seyfiabad Shapouri, M. R., Moatamedi, H., Roayaei, M., Goudarzi, H.. Cloning and secretory expression of VP2 gene of infectious bursal disease virus in eukaryotic cells. , 1388; 11(1): 72-77. doi: 10.22099/ijvr.2010.178

Cloning and secretory expression of VP2 gene of infectious bursal disease virus in eukaryotic cells

Iranian Journal of Veterinary Research
مقاله 10، دوره 11، شماره 1 - شماره پیاپی 30، 2010، صفحه 72-77    اصل مقاله (123.76 K)
نوع مقاله: Short paper
شناسه دیجیتال (DOI): 10.22099/ijvr.2010.178
نویسندگان
Sh. Ghafari1؛ M. R. Seyfiabad Shapouri* 2؛ H. Moatamedi3؛ M. Roayaei3؛ H. Goudarzi4
1Graduated from Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4Department of Avian Medicine, Razi Vaccine and Serum Research Institute, Karaj, Iran
چکیده
VP2 gene coding region of a vaccinal strain (D78) of infectious bursal disease virus (IBDV) was cloned
in a eukaryotic expression vector, pSec Tag2A. The gene was placed downstream of Ig κ chain leader
sequence, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. The
construct pSec Tag2A-VP2 was transfected in COS-7 cell line and the expression and secretion of VP2 was
assessed by dot blotting and antigen capture ELISA. The antibody used in the immunological assays was a
neutralizing monoclonal antibody (1A6) against VP2. Positive reaction with the antibody indicated the
construct was functional with respect to expression and secretion of a native VP2.
کلیدواژه‌ها
Infectious bursal disease؛ IBDV؛ VP2؛ Eukaryotic expression؛ COS-7 cell line
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