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Moradi Bidhendi, S., Khaki, P., Sattari, M., Heidari, M. H.. (1388). Hyperimmune lipopolysaccharide antiserum mediated inhibition of the adherence of E. coli O157:H7 to HEP-2 cells and large intestine of mice. , 11(1), 56-63. doi: 10.22099/ijvr.2010.176
S. Moradi Bidhendi; P. Khaki; M. Sattari; M. H. Heidari. "Hyperimmune lipopolysaccharide antiserum mediated inhibition of the adherence of E. coli O157:H7 to HEP-2 cells and large intestine of mice". , 11, 1, 1388, 56-63. doi: 10.22099/ijvr.2010.176
Moradi Bidhendi, S., Khaki, P., Sattari, M., Heidari, M. H.. (1388). 'Hyperimmune lipopolysaccharide antiserum mediated inhibition of the adherence of E. coli O157:H7 to HEP-2 cells and large intestine of mice', , 11(1), pp. 56-63. doi: 10.22099/ijvr.2010.176
Moradi Bidhendi, S., Khaki, P., Sattari, M., Heidari, M. H.. Hyperimmune lipopolysaccharide antiserum mediated inhibition of the adherence of E. coli O157:H7 to HEP-2 cells and large intestine of mice. , 1388; 11(1): 56-63. doi: 10.22099/ijvr.2010.176

Hyperimmune lipopolysaccharide antiserum mediated inhibition of the adherence of E. coli O157:H7 to HEP-2 cells and large intestine of mice

Iranian Journal of Veterinary Research
مقاله 8، دوره 11، شماره 1 - شماره پیاپی 30، 2010، صفحه 56-63    اصل مقاله (777.6 K)
نوع مقاله: Full paper (Original article)
شناسه دیجیتال (DOI): 10.22099/ijvr.2010.176
نویسندگان
S. Moradi Bidhendi* 1؛ P. Khaki1؛ M. Sattari2؛ M. H. Heidari3
1Department of Microbiology, Razi Vaccine and Serum Research Institute, Karaj, Iran
2Department of Bacteriology, School of Medicine, Tarbiat Modarres University, Tehran, Iran
3Department of Anatomical Sciences, Faculty of Medicine, Shahid Beheshty University of Medical Sciences, Tehran, Iran
چکیده
Escherichia coli O157:H7 is found in cattle farms and can live in the intestine of healthy cattle. Most
cases of human illnesses including nonbloody diarrhea, hemorrhagic colitis and hemolytic uremic syndrome
can be traced, either directly or indirectly, to cattle. One strategy for reducing the risk of Enterohemorrhagic
Escherichia coli (EHEC) infections in human is to reduce the prevalence of infection in cattle. Antiserum
against whole cell of isolated E. coli O157:H7 from cattle showed inhibition of adherence of this strain to
HEP-2 cells and intestine of model mice. The ability of this antiserum in blocking the attachment of bacteria
to HEP-2 cells in 1:1280 titer and to intestine tissue of mice in 1:640 titer are significant. Histology of
intestine tissue confirms our results. The difference between in vivo and in vitro titrations for blocking the
attachment depends on these two different conditions.
کلیدواژه‌ها
Escherichia coli O157:H7؛ Hyperimmune lipopolysaccharide antiserum؛ Inhibition؛ Adherence؛ HEP-2 cells
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