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Nikbakht Brujeni, Gh., Emam, M., Mahmoudzadeh, H., Hamedmonfared, E., Talebnia Jahromi, R., Rezaei, H.. (1388). Typing of Ovar-DRB1 second exon with PCR-RFLP technique in Iranian Shaul Sheep. , 10(3), 250-254. doi: 10.22099/ijvr.2009.1700
Gh. Nikbakht Brujeni; M. Emam; H. Mahmoudzadeh; E. Hamedmonfared; R. Talebnia Jahromi; H. Rezaei. "Typing of Ovar-DRB1 second exon with PCR-RFLP technique in Iranian Shaul Sheep". , 10, 3, 1388, 250-254. doi: 10.22099/ijvr.2009.1700
Nikbakht Brujeni, Gh., Emam, M., Mahmoudzadeh, H., Hamedmonfared, E., Talebnia Jahromi, R., Rezaei, H.. (1388). 'Typing of Ovar-DRB1 second exon with PCR-RFLP technique in Iranian Shaul Sheep', , 10(3), pp. 250-254. doi: 10.22099/ijvr.2009.1700
Nikbakht Brujeni, Gh., Emam, M., Mahmoudzadeh, H., Hamedmonfared, E., Talebnia Jahromi, R., Rezaei, H.. Typing of Ovar-DRB1 second exon with PCR-RFLP technique in Iranian Shaul Sheep. , 1388; 10(3): 250-254. doi: 10.22099/ijvr.2009.1700

Typing of Ovar-DRB1 second exon with PCR-RFLP technique in Iranian Shaul Sheep

Iranian Journal of Veterinary Research
مقاله 7، دوره 10، شماره 3 - شماره پیاپی 28، 2009، صفحه 250-254    اصل مقاله (135.96 K)
نوع مقاله: Full paper (Original article)
شناسه دیجیتال (DOI): 10.22099/ijvr.2009.1700
نویسندگان
Gh. Nikbakht Brujeni* 1؛ M. Emam2؛ H. Mahmoudzadeh3؛ E. Hamedmonfared4؛ R. Talebnia Jahromi4؛ H. Rezaei5
1Department of Pathobiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
2Ph.D. Student in Microbiology, Department of Pathobiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3Department of Animial and Poultry Health and Nutrition, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
4Graduated from Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
5DVM Student, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
چکیده
Several studies have focused on polymorphisms of major histocompatibility complex (MHC) in sheep, Ovar-MHC. This molecule plays a pivotal role in antigen presentation for eliciting immune responses against invading pathogens. The best-characterized genetic control of disease resistance and immune response in animals is associated with MHC. Numerous molecular genetic investigations have been undertaken to detect polymorphisms of MHC genes and their association with resistance to infectious diseases. We have examined Ovar-DRB1 in DNA samples of 82 Shaul Sheep using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) method. Identities of 8 different patterns and 5 distinct DRB1 alleles among Iranian Shaul Sheep have been determined. PCR-RFLP analysis allows rapid identification of Ovar-DRB1 types and enables rapid discrimination between homozygotes and heterozygotes. Data obtained from the present study have revealed that the exon 2 region of Ovar-DRB1 was highly polymorphic in sheep. So PCR-RFLP can be applied to the analysis of this locus.
 
 
کلیدواژه‌ها
MHC؛ Ovar-DRB1؛ PCR-RFLP؛ Shaul
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