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Evaluation of Echinococcus granulosus coproantigens by Dot-blotting in dogs | ||
| Iranian Journal of Veterinary Research | ||
| مقاله 10، دوره 10، شماره 2 - شماره پیاپی 27، 2009، صفحه 166-171 اصل مقاله (77.31 K) | ||
| نوع مقاله: Full paper (Original article) | ||
| شناسه دیجیتال (DOI): 10.22099/ijvr.2009.1670 | ||
| نویسندگان | ||
| M. Keywanloo1؛ G. R. Hashemi Tabar* 2؛ G. R. Razmi3 | ||
| 1Graduated from Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| 2Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| 3Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| چکیده | ||
| Definitive hosts of the Echinococcus granulosus (E. granulosus ) parasite are carnivores such as dogs,wolves and foxes. Detection of this parasite through faecal examination is not possible. In this study, dotblotting test for E. granulosus -specific coproantigens has been evaluated in dog. Three 2–3-month-old puppies were treated with piperazine and then faecal samples were collected as pre-infection samples. Seven days later, hydatid cysts from livers and lungs of sheep were fed to the puppies. Faecal samples were collected weekly for five weeks as post-infection samples. Soluble protein of pre- and post-infection faecal samples were prepared and dot-blotting test was conducted. In parallel experiments, the presence of E.granulosus eggs and also dot-blotting test were evaluated in 15 faecal samples of dogs collected from Razi Veterinary Hospital in Mashhad. For the detection of protein bands in pre-infection and fifth-week postinfection samples, polypeptide profile was analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that incremental spot colours was observed in samples of experimentally infected dogs collected from the first to fifth post-infection weeks. In dot-blot analysis of faecal samples in 15 dogs, 4 samples were positive, and also these four samples were positive for E.granulosus eggs. In SDS-PAGE, one band in pre-infection and four bands in fifth-week post-infection samples were observed. The molecular weight of pre-infection sample of experimentally infected dogs was 16 kDa and the molecular weights of the samples collected five weeks post-infection were 14, 22, 36 and 45 kDa, respectively. In conclusion, the results of this experiment showed that the dot-blotting method does not produce a reliable outcome. For evaluation of the specific coproantigens of E. granulosus in dogs, coproantigen-ELISA test is needed. | ||
| کلیدواژهها | ||
| Echinococcus granulosus؛ Coproantigens؛ Dot-blotting؛ dog | ||
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