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Genetic variation of Garra rufa fish in Kermanshah and Bushehr provinces, Iran, using SSR microsatellite markers | ||
| Molecular Biology Research Communications | ||
| مقاله 5، دوره 2، شماره 3، آذر 2013، صفحه 81-88 اصل مقاله (115.2 K) | ||
| نوع مقاله: Original article | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2013.1665 | ||
| نویسندگان | ||
| Ali Shabani1؛ Ghasem Askari* 1؛ Amin Moradi2 | ||
| 1Department of Fishery, Gorgan University of Agricultural Sciences and Natural Resources, Iran | ||
| 2Marine Sciences and Technology of Khoramshahr University | ||
| چکیده | ||
| Six highly variable microsatellite loci were used to investigate the genetic diversity and population structure of the Garra rufa in Kermanshah and Bushehr provinces, Iran. All of the 6 microsatellite loci screened in this study showed polymorphism. A total of 90 individual fish from 3 populations were genotyped and 60 alleles were observed in all loci. The number of alleles per locus ranged from 6 to14. The average allelic number of these polymorphic markers was 10. The averages of observed (Ho) and expected heterozygosity (He) was 0.529 and 0.826, respectively. The genetic distance values ranged between 0.235-0.570. The UPGMA dendrogram based on genetic distance resulted in three clusters: Gamasiab population alone was classified as one and the other two populations as the second cluster. This study revealed a fairly high level of genetic variation in the microsatellite loci within the three populations, and identified distinct population groups of Garra rufa. This study gains significance for the analysis of the populations’ genetic diversity as well as the management of this important fish resource. | ||
| کلیدواژهها | ||
| Garra rufa؛ Genetic diversity؛ Population؛ Kermanshah؛ Bushehr | ||
| مراجع | ||
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1.Abedi M, Shiva AM, Mohammadi H, Malekpour R. Reproductive biology and determination of Garra rufa Heckel, 1843 (Actinopterygii: Cyprinidae) in Central Iran. Turk J Zool 2011;35:317-323.
2.Askari GH, Shabani A, Kolangi Miandare H. Application of molecular markers in fisheries and aquaculture. Scient J of Animal Sci 2013;2:82-88.
3.Askari GH, Shabani A. Genetic diversity evaluation of Paraschistura bampurensis (Nikolskii, 1900) in Shapour and Berim rivers (Iran) using microsatellite markers. Cell Bio Genet 2013; 3:29-34.
4.Shabani A, Askari GH. Microsatellite loci to determine population structure of Garra rufa (Heckel, 1843) in the Khuzestan Province (Iran). Inter J Aquatic Bio 2013;1:188-194.
5.Matura R, Sharma S, Barat A, Pande V, Mahanta P. Development and characterization of microsatellite markers in Garra gotyla (Family: Cyprinidae, Pisces). Mol Ecol Res 2012; 12:185-189.
6.Ma CY, Cheng QQ, Zhang QY, Zhuang P. Genetic variation of Coilia ectenes (Clupeiformes: Engraulidae) revealed by the complete cytochrome b sequences of mitochondrial DNA. J Exp Mar Biol Ecol 2010;385;14-19.
7.Peakall R, Smouse PE. GenAlex 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinform 2012;28:2537-2539.
8.Raymond M, Rousset F. Genepop (versionb1.2): population genetic software for exact tests and ecumenicism. Heredity 1995;86:248-249.
9.Excoffier L, Laval G, Schneider S. Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evolut Bioinform 2005;1:47-50.
10.Felsenstein J. PHYLIP (Phylogeny Inference Package) Version 3.57c. A Software for Population Genetic Analysis. Department of Genetics, SK-50, University of Washington, Seattle 1995; WA, USA.
11.Dewoody JA, Avise JC. Microsatellite variation in marine, freshwater and anadromous fishes compared with other animals. Fish Biol 2000;56:461-473.
12.Askari GH, Shabani A, Ghodsi Z, Nowferesti H. Genetic comparison of Oxynoemacheilus Kiabii (Golzarianpour, Abdoli & Frehof, 2011) from Gamasiab River in Kermanshah and Hamadan Province, using microsatellite markers. Modern Genet 2013 (In press).
13.Appleyard SA, Grewe PM, Innes BH, Ward RD. Population structure of yellow fin tuna (Thunnus albacares) in the western Pacific Ocean, inferred from microsatellite loci. Mar Biol 2001;139:383-393.
14.Diaz-Jaimes P, Uribe-Alcocer M. Spatial differentiation in the eastern Pacific yellowfin tuna revealed by microsatellite variation. Fish Sci 2006;72:590-596.
15.Ruzzante DE, Taggart CT, Cook D. Spatial and temporal variation in the genetic composition of a larval cod (Gadus morhua) aggregation: cohort contribution and genetic stability. Can J Fish Aquat Sci 1996;53:2695-2705.
16.Nasren S, Islam MN, Quader Khan MG, Islam MS, Alam MS. Genetic variation and differentiation in the Stinging catfish, Heteropneustes fossilis (Bloch), populations assessed by heterologous microsatellite DNA markers. Indian J Biotech 2009; 8: 85-90. | ||
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