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A convenient method to generate methylated and un-methylated control DNA in methylation studies | ||
| Molecular Biology Research Communications | ||
| مقاله 2، دوره 2، شماره 3، آذر 2013، صفحه 57-61 اصل مقاله (155.96 K) | ||
| نوع مقاله: Short communication | ||
| شناسه دیجیتال (DOI): 10.22099/mbrc.2013.1647 | ||
| نویسندگان | ||
| Mehdi Manoochehri* 1؛ Mojgan Bandehpour1؛ Bahram Kazemi2 | ||
| 1Biotechnology department, Shahid Beheshti University of Medical Sciences, Tehran, Iran | ||
| 2Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran | ||
| چکیده | ||
| Methylated and un-methylated control DNA is an important part of DNA methylation studies. Although human and mouse DNA methylation control sets are commercially available, in case of methylation studies on other species such as animals, plants, and bacteria, control sets need to be prepared. In this paper a simple method of generating methylated and un-methylated control DNA is described. Whole genome amplification and enzymatic methylation were performed to generate un-methylated and methylated DNA. The generated DNA were confirmed using methylation sensitive/dependant enzymes, and methylation specific PCR. Control reaction assays confirmed the generated methylated and un-methylated DNA. | ||
| کلیدواژهها | ||
| Epigenetics؛ DNA methylation؛ Control DNA | ||
| مراجع | ||
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