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Salamat, N., Alboghobeish, N., Hashemitabar, M., Mesbah, M., Ahangarpour, A.. (1387). Pituitary primary cell culture of Common carp (Cyprinus Carpio) and evaluation of its secretion effect on endocrine activity of incubated ovarian follicles. , 10(1), 61-65. doi: 10.22099/ijvr.2009.1091
N. Salamat; N. Alboghobeish; M. Hashemitabar; M. Mesbah; A. Ahangarpour. "Pituitary primary cell culture of Common carp (Cyprinus Carpio) and evaluation of its secretion effect on endocrine activity of incubated ovarian follicles". , 10, 1, 1387, 61-65. doi: 10.22099/ijvr.2009.1091
Salamat, N., Alboghobeish, N., Hashemitabar, M., Mesbah, M., Ahangarpour, A.. (1387). 'Pituitary primary cell culture of Common carp (Cyprinus Carpio) and evaluation of its secretion effect on endocrine activity of incubated ovarian follicles', , 10(1), pp. 61-65. doi: 10.22099/ijvr.2009.1091
Salamat, N., Alboghobeish, N., Hashemitabar, M., Mesbah, M., Ahangarpour, A.. Pituitary primary cell culture of Common carp (Cyprinus Carpio) and evaluation of its secretion effect on endocrine activity of incubated ovarian follicles. , 1387; 10(1): 61-65. doi: 10.22099/ijvr.2009.1091

Pituitary primary cell culture of Common carp (Cyprinus Carpio) and evaluation of its secretion effect on endocrine activity of incubated ovarian follicles

Iranian Journal of Veterinary Research
مقاله 10، دوره 10، شماره 1 - شماره پیاپی 26، 2009، صفحه 61-65    اصل مقاله (112.43 K)
نوع مقاله: Full paper (Original article)
شناسه دیجیتال (DOI): 10.22099/ijvr.2009.1091
نویسندگان
N. Salamat* 1؛ N. Alboghobeish1؛ M. Hashemitabar2؛ M. Mesbah3؛ A. Ahangarpour4
1Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2Department of Anatomical Sciences, School of Medicine, Jondishapoor University of Medical Sciences, Ahvaz, Iran
3Department of Aquatic Animal Health and Diseases, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4Department of Physiology, School of Medicine, Jondishapoor University of Medical Sciences, Ahvaz, Iran
چکیده
In this study, five carp pituitary glands were collected and dispersed enzymatically and mechanically.
Then, the cells were cultivated as monolayer in MEM (minimum essential medium Eagle). The culture media
were collected after 72 h and frozen at -20°C. Carp ovarian follicles also were separated mechanically and
incubated in BSS (basic salt solution) Cortland medium in 24-well microplates for 48 h at 20°C. Then, they
were divided into two groups: control group which were incubated in BSS medium and experimental group
which subdivided into three subgroups according to treatment with different concentration of collected
pituitary secretion (50, 100 and 200 μl/ml). Follicles culture media were collected 24 h later and were
analyzed for 17-β-oestradiol (E2) and 17-α-hydroxy progesterone (P4) content by radioimmunoassay (RIA).
The results showed that adding low concentration (50 μl/ml) of collected pituitary secretion (CPS) increased
steroid hormones (E2 and P4) secretion of incubated ovarian follicles significantly (P<0.05) but the high
concentration of CPS (200 μl/ml) significantly decreased the secretion of E2 and P4 (P<0.05). Collected
pituitary secretion at the concentration of 100 μl/ml had no significant effect on steroid hormones (P>0.05).
کلیدواژه‌ها
Pituitary؛ Cell culture؛ Steroids؛ Radio immunoassay؛ Common carp
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