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Jafari Jozani, R., Moghaddam, Gh., Khazraiinia, P., Jabbari Nooghabi, H.. (1389). Adaptation of an indirect enzyme-linked immunosorbent assay by purified gp51SU for detection of antibodies to bovine leukemia virus. , 11(4), 332-336. doi: 10.22099/ijvr.2010.103
R. Jafari Jozani; Gh. Moghaddam; P. Khazraiinia; H. Jabbari Nooghabi. "Adaptation of an indirect enzyme-linked immunosorbent assay by purified gp51SU for detection of antibodies to bovine leukemia virus". , 11, 4, 1389, 332-336. doi: 10.22099/ijvr.2010.103
Jafari Jozani, R., Moghaddam, Gh., Khazraiinia, P., Jabbari Nooghabi, H.. (1389). 'Adaptation of an indirect enzyme-linked immunosorbent assay by purified gp51SU for detection of antibodies to bovine leukemia virus', , 11(4), pp. 332-336. doi: 10.22099/ijvr.2010.103
Jafari Jozani, R., Moghaddam, Gh., Khazraiinia, P., Jabbari Nooghabi, H.. Adaptation of an indirect enzyme-linked immunosorbent assay by purified gp51SU for detection of antibodies to bovine leukemia virus. , 1389; 11(4): 332-336. doi: 10.22099/ijvr.2010.103

Adaptation of an indirect enzyme-linked immunosorbent assay by purified gp51SU for detection of antibodies to bovine leukemia virus

Iranian Journal of Veterinary Research
مقاله 5، دوره 11، شماره 4 - شماره پیاپی 33، 2010، صفحه 332-336    اصل مقاله (107.71 K)
نوع مقاله: Full paper (Original article)
شناسه دیجیتال (DOI): 10.22099/ijvr.2010.103
نویسندگان
R. Jafari Jozani* 1؛ Gh. Moghaddam1؛ P. Khazraiinia2؛ H. Jabbari Nooghabi3
1Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
2Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3Department of Statistics, Faculty of Mathematical Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده
The objective of this study was to compare an indirect ELISA, based on a purified 60 kDa envelope
glycoprotein (gp51SU), with a Pourquire indirect ELISA for the detection of antibodies to the bovine
leukemia virus. For conducting this research, 340 serum samples were collected from two different breeds of
cows (Sarabi and Holestin) in different herds. Commercial ELISA revealed positive results in 17 (7%)
Holstein cows. An appropriate ELISAcut-off was determined by receiver operating curve (ROC) analysis in
comparison with commercial indirect ELISA. Results showed a relative sensitivity and specificity of 97%
and 92%, respectively, for a cut-off value of 0.34 in the domestic ELISA. In conclusion, the results of the
present study showed that domestic developed kit can be used for diagnosis of bovine leukemia virus with
appropriate sensitivity and specificity. In addition, a comparison of the results from a native breed, Sarabi,
with Holstein showed that there was no significant (P>0.05) difference in the frequency of infection with
BLV between the two breeds.
کلیدواژه‌ها
Bovine leukemia virus (BLV)؛ gp51SU؛ ELISA؛ Relative specifity؛ Relative sensitivity
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